Composite

Part:BBa_K2150115

Designed by: Zepeng Mu   Group: iGEM16_UCAS   (2016-10-13)


6249 antitoxin tet

This antitoxin belongs to ParD family. It can effectively neutralize the toxin ParE. We characterized this by measuring the growth curve co-expressing toxin and antitoxin.

This protein is induced by aTc. When studying the interaction between antitoxin and toxin, antitoxin is induced by IPTG.

See: BBa_K2150117

We also characterized its corresponding toxin, ParE, see: BBa_K2150111

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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